ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Objective To determine the antibacterial activity of propolis from Henan province against Bacillus anthracis. Methods The agar diffusion method was used to test for antibacterial activity. Antibacterial effects were measured after exposure to seven formulations of 31% propolis at pH ranging from 5.5-8.5 (5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5). Additionally, ten dilutions of propolis from 31% to 0.06%(1∶512), including a 0 control, at pH 5.5 and a dose of 7 μl were examined in triplicate. Results The diameters (mm) of the zones of inhibition in each pH group, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0 and 8.5, were as follows: 19.16±0.29, 17.67±0.29, 14.67±0.58, 13.67±0.29, 14.00±0.00, 13.33±1.15 and 12.00±0.00 mm, respectively. The diameters in each concentration group, 15.50%, 7.75%, 3.88%, 1.94%, 0.97%, 0.48%, 0.24%, 0.12% and 0.06%, and the control group were: 19.50±1.80, 19.67±1.04, 17.75±0.35, 15.67±0.58, 14.00±2.29, 13.17±1.04, 11.83±1.53, 10.83±1.26, 9.00±0.00 and 0.00±0.00 mm, respectively. Conclusion Propolis from Henan province had antibacterial activity against B. anthracis, which declined with increasing pH or decreasing concentration.
【Abstract】 Objective To investigate the effect of high temperature on antibacterial activity of antibacterial substances induced in the larvae of house fly by pricking. Methods The induced hemolymph was bathed at 100 ℃ for 10 sec, 30 sec, 1 min, 3 min, 5 min, 80 ℃ and 60 ℃ for 30 sec, 1 min, 3 min, 5 min, 10 min, then solved in the buffer solution of Na2HPO4?KH2PO4(1/15 mol/L,pH 6.5) and centrifuged, the supernatant was used to test the bacteriostasic efficiency to Micrococcus lysodeikticus and Staphylococcus aureus respectively. Results Diameter of antibacterial ring against M.lysodeikticus decreased along with the increase of bath time at both 100 ℃ and 80 ℃. No antibacterial ring against S.aureus was found after bathed at 100 ℃ for 1 min or 80 ℃ for 3 min. Diameters of antibacterial ring were similar after bathed at 60 ℃ for different time against both M.lysodeikticus and S.aureus. Conclusion It suggested that the antibacterial activity of antibacterial substance induced by pricking in the house fly was stable against high temperature in a certain extent. Antibacterial activity weakened along with the increase of bath time at 100 ℃ and 80 ℃, and no obvious change after bathed at 60 ℃ for 10 s-10 min.
【Abstract】 Objective To survey reservoir hosts of desert-type kala-azar in Tarim Basin. Methods Collect animal samples in winter, and screen the samples which antibody were positive by ELISA. Leishmania was isolated by Lagurus lagurus inoculation and tissue culture. The specific gene sequences of Leishmania infantum isolated from kala?azar patients, Yarkend hare and Phlebotomus wui were amplified by molecular biological technology. Results There was Leishmania antibodies in Yarkend hare and Canis familiaris, and 3 strains of Leishmania were isolated from 44 of Yarkend hare which antibodies were positive. The NAGT gene sequences amplified from hares (3 strains), patients (1 strain) and vectors (6 strains) were same, according with the sequence of L.infantum (AF205934) from GenBank. Conclusion It suggests that yarkend hare is one of the primal host of desert-type kala-azar in Tarim Basin.